Bioluminescent imaging system

  • IVIS Spectrum from Perkin Elmer, coupled to Minihub gas anesthesia module from Anesteo
  • Located in BSL2 zone of Purpan animal facility (INSERM UMS006)
  • The IVIS Spectrum allows imaging and 3D-reconstruction of bioluminescent and fluorescent signals in anesthesized small rodents or explanted tissues infected with BSL2 microorganisms. This sensitive device will facilitate non-invasive longitudinal monitoring of pathogen dissemination (e.g. genetically modified to express luciferase or fluorescent reporters) concomitantly to immune responses and inflammatory status.

Local contact: Dr Nicolas Blanchard - E-mail: nicolas.blanchard@inserm.fr

Physiological and behavioral core

The physiological and behavioral core of ANINFIMIP allows the longitudinal evaluation of multiple parameters in mice and rats. This equipment has been installed in the BSL2 animal facility at Purpan hospital. Two different systems of analysis are available:

  • For Motor and Behavioral analysis, the platform offers 12 different systems to assess various parameters, including: motor coordination (rotarod), anxiety (elevated plus-maze), activity (actimeter), food and water intake (metabolic cages) and sensory pain (von Frey, Plantar test).

Local contact: Dr Daniel Dunia - E-mail: daniel.dunia@inserm.fr

  • For In vivo monitoring, the platform offers data acquisition and recording elements (BioAmp, Powerlab) to assess various parameters such as temperature, blood pressure, EMG, ECG, EEG.

Local contact: Dr Nicolas Cenac - E-mail: nicolas.cenac@inserm.fr

Multichromatic flow cytometry and cell sorting

At CPTP

  • Equipments: 2 MACSQuants (Miltenyi Biotec) MACSQuant 10: 3 lasers (405nm, 488nm, 633nm) 8 fluorescences / MACSQuant VYB : 3 lasers (405nm, 488nm, 561nm) 8 fluorescences
  • Location: Cytometry and Cell sorting core facilty of the CPTP
  • Applications: Flow cytometry allows to determine the phenotype and functions of cell populations directly harvested from infected or vaccinated animals, without the need for chemical fixation, which commonly hapers phenotypic analysis. More precisely, these machines allow the analysis of membrane and intracellular multi-labeling as well as cell cycle, apoptosis and transfection rate.

Local contacts: Fatima L’Faqihi - E-mail: fatima.lfaqihi@inserm.fr & Valérie Duplan - Email: valerie.duplan@inserm.fr

At ENVT

  • Equipments: MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotech)
  • Location: Toulouse Veterinary College (INP-ENVT), UMR1225 Host-Pathogen Interactions (IHAP), Building 10.3/Immunology section
  • Applications: The flow cytometer MACSQuant is equipped with three lasers (blue, red, purple) and allows the simultaneous analysis of 10 optical parameters, including eight channels of fluorescence. Analysis ofany cell suspension in small to large volumesis possible. Ahigh throughput system enables acquisition of series of a large number of samples in 96-well plates. Absolute quantification of the event concentration is included, andpre-enrichment of population of interest is availableon request.

Local contact: Gilles Foucras - E-mail: g.foucras@envt.fr

At IPBS

  • Equipments: 1 MACSQuant (Miltenyi Biotec) 8 fluorescences & 1 FACS Aria Fusion 12 colors (BD)
  • Location: BSL3 animal facility at IPBS
  • Applications: Flow cytometry & cell sorting allow to determine the phenotype and functions of cell populations (prokaryotic or eukaryotic) directly harvested from infected or vaccinated animals, without the need for chemical fixation, which commonly hampers phenotypic analysis and subsequent functional assays.

Local contact: Antonio Peixoto - E-mail: antonio.peixoto@ipbs.fr

Fluorescence microscopy & live imaging

  • Equipments: 1 bi-photon microscope (Lavision Biotech)
  • Location: BSL3 animal facility at IPBS
  • Applications: Fluorescence microscopy analysis of infected cells and tissues.

Local contact: Antonio Peixoto - E-mail: antonio.peixoto@ipbs.fr